Safety Data Sheets / Trypan Blue, C.I. 23850
Safety Data Sheet
Trypan Blue, C.I. 23850
1. Identification
Product Identifiers
- Product Name
- Trypan Blue, C.I. 23850
- Other Names
- C.I. 23850; Direct Blue 14; Niagara Blue 3B.
- Product Number(s)
- C091, C0915
- CAS Number
- 72-57-1
Recommended use of the chemical and restriction on use
- Laboratory use.
- Biological Stain.
Company Details
ProSciTech Pty Ltd
11 Carlton Street
KIRWAN QLD 4817
Australia
(07) 4773 9444
pst@proscitech.com
proscitech.com.au
11 Carlton Street
KIRWAN QLD 4817
Australia
(07) 4773 9444
pst@proscitech.com
proscitech.com.au
Emergency Contact Details
ProSciTech Pty Ltd
11 Carlton Street
KIRWAN QLD 4817
Australia
(07) 4773 9444
pst@proscitech.com
proscitech.com.au
11 Carlton Street
KIRWAN QLD 4817
Australia
(07) 4773 9444
pst@proscitech.com
proscitech.com.au
2. Hazards Identification
Classification of the substance or mixture
Carcinogenicity (Category 2)
Label Elements

- Signal Words
- None
- Hazard Statement(s)
- H350: May cause cancer
- Precautionary Statement(s)
- P201: obtain special instructions before use.
- P202: Do not handle until all safety precautions have been read and understood.
- P405: Store locked up.
- P281: Use PPE as required.
- P308 ±P313: If exposed or concerned: contact doctor/physician.
- P501: Dispose of contents/container to an approved waste disposal plant.
Primary route(s) of entry
Not available.
Human Health
- Inhalation
- Harmful by inhalation.
- Ingestion
- Harmful if swallowed.
- Eyes
- Risk of serious damage to eyes.
- Skin
- Harmful in contact with skin.
Environment
Not available.
3. Composition / Information on Ingredients
| Name | CAS No. | Content (w/w) | Classification |
|---|---|---|---|
| Tetrasodium 3,3'-[(3,3'-dimethyl[1,1'-biphenyl]-4,4'-diyl)bis(azo)] bis[5-amino-4-hydroxynaphthalene | 72-57-1 | 100% | - |
4. First Aid Measures
- Ingestion
- Do not induce vomiting without medical advice. Rinse with water. Consult physician.
- Inhalation
- Move to fresh air. Consult a physician.
- Skin Contact
- Rinse with plenty of soap & water and seek medical advice.
- Eye Contact
- Rinse immediately with plenty of water and seek medical advice.
- Other Information
- Treat symptomatically and supportively.
5. Firefighting Measures
Suitable extinguishing equipment
- Fire
- Use water spray, dry chemical, carbon dioxide, or alcohol-resistant foam.
- HAZCHEM
- Not available.
Special protective equipment and precautions for fire fighters
As in any fire, wear a self-contained breathing apparatus in pressure-demand, MSHA/NIOSH (approved or equivalent), and full protective gear. During a fire, irritating and highly toxic gases may be generated by thermal decomposition or combustion.
6. Accidental Release Measures
- Personal precautions, protective equipment and emergency procedures
- Use proper PPE as indicated in Section 8.
- Environmental precautions
- Avoid runoff into storm sewers and ditches which lead to waterways.
- Do not let product enter drains.
- Prevent further leakage or spill is safe to do so.
- Methods and materials for containment and clean up
- Clean up spills immediately, observing precautions in the Protective Equipment section. Sweep up, then place into a suitable container for disposal. Avoid generating dusty conditions. Provide ventilation.
7. Handling and Storage
- Precautions for safe handling
- Wash thoroughly after handling. Wash hands before eating. Remove contaminated clothing and wash before reuse. Use with adequate ventilation. Avoid contact with eyes, skin, and clothing. Keep container closed when not in use. Store in a tightly closed container. Store in a cool, dry, well-ventilated area away from incompatible substances.
- Conditions for safe storage
- Keep container closed when not in use. Store in a tightly closed container. Store in a cool, dry, well-ventilated area away from incompatible substances.
8. Exposure Controls / Personal Protection
Exposure Standards
- Engineering controls
- Eyebath/eyewash & Safety shower.
- Use process enclosure, local exhaust ventilation, or other engineering controls to control airborne levels.
Personal protective equipment
- Eye and face protection
- Safety glasses.
- Skin protection
- The selected protective gloves have to satisfy the specifications of EU Directive 89/686/EEC and the standard EN 374 derived from it. Handle with gloves. 0.11mm thick nitrile.
- Body protection
- Choose body protection according to the amount and concentration of the dangerous substance at the work place.
- Hygiene measures:
- Avoid contact with skin, eyes and clothing. Wash hands before breaks and immediately after handling the product.
- Respiratory protection
- Where risk assessment shows air-purifying respirators are appropriate use a full-face particle respirator type N99 (US) or type P2 (EN 143) respirator cartridges as a backup to engineering controls. If the respirator is the sole means of protection, use a full-face supplied air respirator. Use respirators and components tested and approved under appropriate government standards such as NIOSH (US) or CEN.
9. Physical and Chemical Properties
General information
- Appearance
- Dark blue to black powder.
- Odour
- Ordorless.
- pH
- Not available.
- Vapour Pressure
- Not available.
- Density
- Not available.
- Boiling Point
- Not available.
- Melting Point
- >300°C (>572°F)
- Solubility
- Not available.
- Specific Gravity of Density
- Not available.
- Flash Point
- Not available.
- Flammable (Explosive) Limits
- Not available.
- Ignition Temperature
- Not available.
- Formula
- C34H24N6Na4O14S4
10. Stability and Reactivity
- Reactivity
- No further relevant information available.
- Chemical stability
- Stable under normal temperatures and pressures.
- Possibility of hazardous reactions
- No further relevant information available.
- Conditions to avoid
- Incompatible materials, dust generation, excess heat, strong oxidants.
- Incompatible materials
- Oxidizing agents.
11. Toxicological Information
- Acute effects
- No further relevant information available.
- Eye contact
- No further relevant information available.
- Skin contact
- No further relevant information available.
- Ingestion
- No further relevant information available.
- Inhalation
- No further relevant information available.
- Toxicity and irritation
- LD50/LC50:
- CAS# 72-57-1:
- Oral, rat: LD50 = 6.200 mg/kg;
12. Ecological Information
- Ecotoxicity
- LC50-Oryzias latipes >1,000mg/l-48h
- Persistence and degradability
- No further relevant information available.
- Bioaccumulative potential
- No further relevant information available.
- Other adverse effects
- No further relevant information available.
13. Disposal Considerations
General information
Chemical waste generators must determine whether a discarded chemical is classified as a hazardous waste. US EPA guidelines for the classification determination are listed in 40 CFR Parts 261.3. Additionally, waste generators must consult state and local hazardous waste regulations to ensure complete and accurate classification. Contact a licensed professional waste disposal service to dispose of this material.
14. Transport Information
ADG label required
- HAZCHEM
- Not available.
- UN Number
- UN307
- Proper shipping name
- (Tetrasodium 3,3'-[(3,3'-dimethyl[1,1'-biphenyl]-4,4'-diyl)bis[5-amino-4-hydroxynaphthalene-2,7-disulphonate])
- Transport hazard class
- 0
- Packing group
- None.
- Environmental hazard
- Yes
- Special precautions for users
- Not regulated.
- Additional information
- No information available.
15. Regulatory Information
- Poisons Schedule Number
- Not listed.
- Other Information
- No further relevant information available.
16. Other Information
- SDS preparation date
- 2 March 2026
- Comments
- This information is based on our present knowledge. However, this shall not constitute a guarantee for any specific product features and shall not establish a legally valid contractual relationship.
